Heartiness crosses are looking good, though developmental stages are somewhat spread. (The flies in several vials from set 1 have fully eclosed.) Next step is to collect virgins from each F1 to set up parental pool for F2 cages -- should be collecting virgins every day for the next week or until enough parents are collected, whichever comes first.
A few weeks back I was trying to kill off a vial of flies to use to determine the angle of the inclines in the "fractionator" tower -- I stuck these flies into a ~(-20 deg C) freezer for a little over an hour and left them without food for three days. By the end of day three each and every fly was still alive and kicking. To test this unexpected resilience, Alan and I designed a simple experiment: take vials of 10 females from each strains and subject them to one of 6 exposure lengths to -20 deg C (control (o min), 15 min, 30 min, 60 min, 120 min, 240 min). I'm planning to make a pilot run for this experiment with two strains (one heat resistant, one heat sensitive) sometime before break.
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